Part:BBa_K358019
Lysis cassette regulated by lacP
Lysis cassette[SRRz] is regulated by lactose promoter. Activating lactose promoter and expressing SRRz gene, it causes the cell lysis. So, lactose promoter must be repressed when transform this part.
To repress lactose promoter tightly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX, not Top 10. KRX has lacI in its genome and Top 10 hasn't (see genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf],[http://openwetware.org/wiki/E._coli_genotypes#TOP10_.28Invitrogen.29]).
Added by THINKER_CHINA
Procedures to prove our lysis module using lac promoter:
1. Cultivate E. Coli in LB mediums at 37 degrees Celsius and 200 rpm.
2. When OD600 values equals to 0.4, add different concentrations of copper, three times for each group.
3. Measure OD600 values at 0.5h, 1h, 1.5h, 2h, 3h and 4h intervals.
the graph suggested that the lysis circuit works regularly when the concentration of copper is above 10^-5 mol/L.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
n/a | Lysis cassette regulated by lacP |